Western blot analysis of PFKFB3 in extracts of various cell lines. Samples were incubated with PFKFB3 Monoclonal antibody (Product # MA5-35419) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot analysis of PFKFB3 performed on various cell lines using a PFKFB3 monoclonal antibody (Product # MA5-35419) at a dilution of 1:500. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.
Supplier Page from Thermo Fisher Scientific for PFKFB3 Antibody